Radioimmunoassay that measures serum vitamin B12.

Radioimmunoassay that measures serum vitamin B12.

AIMS To develop a specific radioimmunoassay for the routine determination of serum vitamin B12. METHODS Antisera were raised in rabbits by immunisation with the monocarboxylic acid derivative of cyanocobalamin coupled to human serum albumin. Antibody titres and affinities were determined and the antiserum giving the highest binding affinity constant, Ka, was used to develop the assay protocol...

A solid-phase radioimmunoassay for vitamin B12 in serum, with use of radioiodinated tyrosine methyl ester of vitamin B12.

Although radioassays for vitamin B12 with use of any of several binding proteins have been available for many years, a radioimmunoassay for B12 has not been reported. We describe here such a radioimmunoassay, incorporating, for the first time, a radioiodinated tyrosine methyl ester of B12 as the radioactive tracer. Polypropylene tubes are coated with antiserum raised in a rabbit against B12/bov...

Clinical application of serum vitamin B12 assay.

Serum Acylcarnitines and Vitamin B12 Deficiency, Mark

Serum Acylcarnitines and Vitamin B12 Deficiency, Mark M. Kushnir, Bori Shushan, William L. Roberts, and Marzia Pasquali ( ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108; 2 MDS SCIEX, Concord, ON, L4K 4V8 Canada; 3 Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84132; * author for correspondence: fax 801-584-5207, email kushnm...

Interlaboratory comparison of serum vitamin B12 assay.

The results have been compared of microbiological and radioisotope dilution (RID) assay of serum vitamin B12 by participants in national interlaboratory trials in Britain. There was wide variation between the individual participants, especially marked in the L. leichmannii microbiological assay and in the RID methods, whereas excellent correlation, reproducibility, and recovery were obtained in...